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Merck

G5768

Sigma-Aldrich

Gamborg's B-5 基本混合盐

fine powder, suitable for plant cell culture

别名:

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.72

一般說明

由Gamborg等描述的Gamborg′B-5培养基可以完全培养基(G5893)进行购买或通过从Vitamin 1000x(G1019)和Basal Salt混合物(G5768)等化学品储备物进行重悬而得。 使用Gamborg′B-5(B5)培养基用于植物细胞系和组织的体外培养,如墨西哥红豆杉、大壶藓、白亚麻、赤桉。

應用

Gamborg′s B-5基础盐混合物已被用于:
  • 制备TEX缓冲液的组分
  • 作为培养基维持马铃薯悬浮及愈伤组织培养
  • 作为主茎节变形方法的培养基

Gamborg′B-5培养基已被用于培养番荔枝愈伤组织。

公式變體

如Gamborg等(1968)所描述的,含有大量和微量营养素的浓度。

培养基配方

數量

配制成每升培养基含 3.1 克粉末。

象形圖

Flame over circle

訊號詞

Warning

危險聲明

危險分類

Ox. Sol. 3

儲存類別代碼

5.1B - Oxidizing hazardous materials

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and

商品

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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