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運輸包裝
wet ice
儲存溫度
−20°C
應用
黑附球菌酮(epicocconone)荧光团与蛋白质可逆共价结合，为蛋白质定量提供了一种强大、灵敏的方法
特點和優勢
- 蛋白质结合后会发出强烈的红色荧光
- 结合是完全可逆的，因而使所获得蛋白质适于质谱和功能性检测
- 无需采用有机溶剂，即可定量水溶性蛋白质。
- 动态范围广、迁移行程大、灵敏度高
- 可高度容忍通常会干扰蛋白定量的物质
- 可生物降解、环保
其他說明
通过一种独特的荧光团与多肽的胺相互作用，定量溶液中的总蛋白。
法律資訊
FluoroProfile is a trademark of Sigma-Aldrich Co. LLC
Phillip J L Bell et al.
Journal of the American Chemical Society, 125(31), 9304-9305 (2003-08-02)
Epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus Epicoccum nigrum. Epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long Stokes' shift that will
Ondřej Kaplan et al.
PloS one, 11(10), e0163697-e0163697 (2016-10-08)
We describe the production of a highly-active mutant VEGF variant, α2-PI1-8-VEGF121, which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α2-PI1-8-VEGF121 gene was synthesized, cloned into a pET-32a(+) vector and
R P Ubrihien et al.
Archives of environmental contamination and toxicology, 82(4), 520-538 (2022-04-21)
The effects of multigenerational Cu exposure on the freshwater gastropod Isidorella newcombi were investigated. Snails were exposed to a range of treatment-specific Cu concentrations in the parental to F2 generations, and a common Cu concentration in the F3 generation. In
Helena A Schmitz et al.
Aquatic toxicology (Amsterdam, Netherlands), 160, 22-30 (2015-01-13)
Metals are accumulated by filter feeding organisms via water, ingestion of suspended sediments or food. The uptake pathway can affect metal toxicity. Saccostrea glomerata were exposed to cadmium through cadmium-spiked suspended sediments (19 and 93μg/g dry mass) and cadmium-enriched phytoplankton
Anne M Taylor et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 166, 34-43 (2014-07-11)
The metalloid selenium is an essential element which at slightly elevated concentrations is toxic and mutagenic. In Australia the burning of coal for power generation releases selenium into estuarine environments where it accumulates in sediments. The relationship between selenium exposure
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