EC600

GenElute^™-E Single Spin DNA Cleanup Kit

• 别名: E Single Spin DNA Cleanup Kit, GenElute^™ DNA Cleanup Kit
• 分類程式碼代碼: 41105600
• NACRES: NA.52

属性

• 純化經由: (Single-spin negative chromotography); (Time: 3 minutes or less)
• 特點: Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS); Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions); Typical/expected yield (Varies by sample. Please reference user guide for more information.)
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 技術: DNA purification: suitable
• 環保替代類別: Aligned,
• 儲存溫度: room temp

说明

一般說明

The GenElute^™-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

應用

Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute^™-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

特點和優勢

Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

儲存和穩定性

Use within 12 months of receipt.

其他說明

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute^™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute^™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute^™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3