跳转至内容
Merck

E7034

Sigma-Aldrich

EX-527

≥98% (HPLC)

别名:

6-氯-2,3,4,9-四氢-1H-咔唑-1-甲酰胺

登录查看公司和协议定价


About This Item

经验公式(希尔记法):
C13H13ClN2O
CAS号:
分子量:
248.71
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.77

化驗

≥98% (HPLC)

形狀

powder

顏色

white to beige

溶解度

DMSO: >20 mg/mL

儲存溫度

2-8°C

SMILES 字串

NC(=O)C1CCCc2c1[nH]c3ccc(Cl)cc23

InChI

1S/C13H13ClN2O/c14-7-4-5-11-10(6-7)8-2-1-3-9(13(15)17)12(8)16-11/h4-6,9,16H,1-3H2,(H2,15,17)

InChI 密鑰

FUZYTVDVLBBXDL-UHFFFAOYSA-N

應用

EX-527用于:
  • 1%二甲基亚砜,30% 聚乙二醇400和1%Tween 80中,通过处理C57BL/6N小鼠,以研究其对肠道形态变化和隐窝细胞凋亡的影响
  • 作为sirtuin 1的抑制剂,用于处理在Dulbecco′s改良Eagle′s培养基中培养的人癌症细胞系MCF-7(Michigan cancer foundation-7)和HCT116(结肠癌细胞系),以研究其对线粒体ATP(三磷酸腺苷)产生的影响
  • 脑室内注入大鼠癫痫发生模型,以获取海藻酸诱导的癫痫持续状态所刺激的sirtuin 1活性

生化/生理作用

EX-527是一种有效的选择性sirtuin 1(SIRT1)抑制剂 (IC50 38 nM),可从高通量筛选中鉴定出来。与SIRT2或SIRT3 相比,EX-527 对 SIRT1的选择性更高(200-500 倍),并且已经证明是一种使用H3K56 去乙酰化位点底物的有效 SIRT6抑制剂。EX-527在浓度高达 100uM 时不会抑制 I/II 类 HDAC 活性。 增强 p53 乙酰化以响应 DNA 损伤剂。EX-527是外消旋的;活性异构体(EX-243)给出类似的结果和效力,而另一种异构体(称为EX-242)是无活性的。

特點和優勢

该化合物是基因调控研究的推荐产品。点击此处查看更多特色基因调控产品。在sigma.com/discover-bsm 上了解有关生物活性小分子在其他研究领域的更多信息。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Hong-Xia Liu et al.
Experimental cell research, 357(2), 271-281 (2017-05-30)
Mitochondrial trifunctional protein α-subunit (MTPα) is involved in the fatty acid β-oxidation (FAO) pathway. Two MTPα activities, 3-hydroxyacyl-CoA dehydrogenase and long-chain hydratase, have been linked with the occurrence and development of obesity and obesity-related disorders. These activities catalyze two steps
A Rise in ATP, ROS, and Mitochondrial Content upon Glucose Withdrawal Correlates with a Dysregulated Mitochondria Turnover Mediated by the Activation of the Protein Deacetylase SIRT1
Song S and Hwang E
Cells, 8(1), 11-11 (2019)
Takahito Abe et al.
The Journal of reproduction and development, 63(5), 455-461 (2017-06-13)
Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism and ATP generation in somatic cells. In the present study, the effects of supplementing culture medium with resveratrol on lipid metabolism, ATP generation, and cryosensitivity of
The Role of Sirt1 in Epileptogenesis
Hall Alicia M, et al.
eNeuro, 4(1) (2017)
The protective effects of Resveratrol against radiation-induced intestinal injury
Zhang H, et al.
BMC Complementary and Alternative Medicine, 17(1), 410-410 (2017)

商品

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门