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重組細胞
expressed in HEK 293 cells
化驗
≥98% (SDS-PAGE)
形狀
lyophilized powder
效力
≤2.5 ng/mL ED50 (measured in a cell proliferation assay using TF-1 human cells)
技術
cell culture | mammalian: suitable
雜質
Endotoxin, tested
儲存溫度
−20°C
一般說明
Recombinant human erythropoietin (EPO) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of ~18 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The protein is produced with no artificial tags.
生化／生理作用
Erythropoietin (EPO) is a glycoprotein hormone, known for its erythropoietic activity. It is used as treatment for anemia in humans with renal failure or cancer. EPO also reduces the amount of blood transfusions required on premature infants and surgeries.
EPO consists of 165 amino acids and has a high carbohydrate content, of both N-linked and O-linked types. The oligosaccharide chains are responsible for EPO production, secretion, longevity, and functioning. The biological activity of EPO depends upon two disulfide bonds, one formed between cysteine7 and cysteine160, and the other between cysteine29 and cysteine33. Thus, EPO proper folding, glycosylation and disulfide bonds formation are crucial for its function.
EPO was found to downregulate HIF-1α in the diabetic rat model. EPO helped maintaining the homeostasis of intracellular zinc in retinal cells, altered as a result of diabetes, by restoring Zinc transporter 8 (ZnT8) expression.
EPO consists of 165 amino acids and has a high carbohydrate content, of both N-linked and O-linked types. The oligosaccharide chains are responsible for EPO production, secretion, longevity, and functioning. The biological activity of EPO depends upon two disulfide bonds, one formed between cysteine7 and cysteine160, and the other between cysteine29 and cysteine33. Thus, EPO proper folding, glycosylation and disulfide bonds formation are crucial for its function.
EPO was found to downregulate HIF-1α in the diabetic rat model. EPO helped maintaining the homeostasis of intracellular zinc in retinal cells, altered as a result of diabetes, by restoring Zinc transporter 8 (ZnT8) expression.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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