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Merck

D5652

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

别名:

DPBS

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
eCl@ss:
32129211
NACRES:
NA.25

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

pH值

7.2-7.8

溶解度

water: 9.6 g/L, clear

儲存溫度

2-8°C

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一般說明

杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。

應用

在制备和连续转移过程中,可使用D-PBS清洗细胞。
杜氏磷酸盐缓冲液已用于:
  • 作为模拟生理溶液成分,体外研究Fe–20Mn–1.2C合金的降解行为
  • 溶解朝鲜当归(Angelica gigas)粉末进行微囊化
  • 在细胞培养物处理、愈伤试验、transwell试验和凋亡试验中用于清洗细胞
  • 洗涤羊膜用于细胞分析

生化/生理作用

杜氏磷酸盐缓冲液可维持细胞渗透压平衡,作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后,这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。

數量

按照每升培养基9.6克粉末配制。

其他說明

该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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