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Merck

D5042

Sigma-Aldrich

123 bp DNA分子量标准

for DNA electrophoresis

别名:

DNA分子量标准, 用于 DNA 电泳的 DNA 梯状标志

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About This Item

分類程式碼代碼:
41105335
NACRES:
NA.25

品質等級

100
200

形狀

liquid

用途

100 uses (0.1 mg size)
250 uses (250 μg size)

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

應用

123 bp DNA 分子量标准已被用作随机扩增多态性 DNA-聚合酶链式反应 (PCR) 分析中的大小标记。它也被用作凝胶电泳的分子量标准。

成分

Sigma 的 123 bp DNA 分子量标准是在 10 mM Tris-HCl (pH 7.5)、50 mM NaCl 和 0.1 mM EDTA 溶液中提供的。

其他說明

包含35个片段,123-4182 bp
为实现最佳分辨率,建议采用的琼脂糖凝胶浓度为1.5%。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and
Sabrina Bochicchio et al.
International journal of pharmaceutics, 525(2), 377-387 (2017-02-13)
Tailored developed nanoliposomes loaded with a siRNA against the transcription factor E2F1 (siE2F1), were produced and delivered to human colorectal adenocarcinoma cell lines and to intestinal human biopsies. siE2F1 loaded nanoliposomes were produced through a dedicated ultrasound assisted technique producing

商品

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

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