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應用
小牛胸腺DNA(CT-DNA)是一种天然DNA,广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。
品質
“高度聚合”
物理性質
λmax 259 nm(100 mM磷酸盐缓冲液,pH7.0)。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0,对应于约50 μg的双链DNA。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0,对应于约50 μg的双链DNA。
外觀
纤维状制剂
準備報告
本品采用未指定性别的小牛的胸腺组织制备。
该产物采用可引起剪切的方法进行提取,产生双链和单链DNA的高度聚合混合物。 但是,双链DNA是主要形式。
重構
DNA溶液已在4℃,pH 7.5-8,含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度(μg/ml)条件下,DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中,因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切,不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜,以便完全溶解DNA。 建议添加1 mM EDTA,以防止核酸酶降解DNA。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
Enzymatic Assay of Nuclease S1, Product No. N5661
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