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Key Documents

D1501

Sigma-Aldrich

脱氧核糖核酸 钠盐 来源于小牛胸腺

Type I, fibers

别名:

ctDNA, 胸腺核酸 钠盐, 脱氧核糖核酸 钠盐 来源于小牛胸腺

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
41106305
eCl@ss:
32160414
NACRES:
NA.51

種類

Type I

品質等級

形狀

fibers

顏色

white

儲存溫度

2-8°C

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應用

小牛胸腺DNA(CT-DNA)是一种天然DNA,广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。

品質

“高度聚合”

物理性質

λmax 259 nm(100 mM磷酸盐缓冲液,pH7.0)。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0,对应于约50 μg的双链DNA。

外觀

纤维状制剂

準備報告

本品采用未指定性别的小牛的胸腺组织制备。
该产物采用可引起剪切的方法进行提取,产生双链和单链DNA的高度聚合混合物。 但是,双链DNA是主要形式。

重構

DNA溶液已在4℃,pH 7.5-8,含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度(μg/ml)条件下,DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中,因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切,不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜,以便完全溶解DNA。 建议添加1 mM EDTA,以防止核酸酶降解DNA。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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M S Hazelton et al.
Journal of dairy science, 101(8), 7412-7424 (2018-05-14)
With the common use of bulls for breeding following a period of artificial insemination in seasonally bred dairy herds, it is important to consider the potential role of the bull in transmission of Mycoplasma spp. within and between herds. This
Alysia M Parker et al.
Veterinary microbiology, 196, 118-125 (2016-12-13)
Mycoplasma bovis is a major pathogen in cattle causing mastitis, arthritis and pneumonia. First isolated in Australian cattle in 1970, M. bovis has persisted causing serious disease in infected herds. To date, genetic analysis of Australian M. bovis isolates has
Hironobu Ikehata et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(4), 404-413 (2018-02-22)
The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot
Oskar Szczepaniak et al.
Biomolecules, 10(5) (2020-05-06)
Since ancient times, fruits and edible plants have played a special role in the human diet for enhancing health and maintaining youthfulness. The aim of our work was to determine the interactions between naringin, a natural ingredient of grapefruits, and
A M Parker et al.
Journal of dairy science, 99(12), 9875-9884 (2016-10-04)
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification

实验方案

Enzymatic Assay of Nuclease S1, Product No. N5661

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