CWA-1064

Anti-Human CD3ε (APA1/1) ColorWheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: APA1/1, monoclonal
• 產品線: ColorWheel^®
• 形狀: lyophilized
• 分子量: calculated mol wt 23.15 kDa
• 物種活性: human
• 物種活性（以同源性預測）: mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 技術: flow cytometry: suitable
• 同型: IgG1
• 表位序列: C-terminal half.
• Protein ID登錄號: NP_000724.1
• UniProt登錄號: P07766
• 相容性: for use with ColorWheel^® Dyes (Required, sold separately)
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CD3E(916)

说明

一般說明

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

特異性

Clone APA1/1 is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD3 epsilon chain (CD3 epsilon). It targets an epitope within the activation motif.

免疫原

SDS denatured CD3 epsilon from human thymus.

應用

Quality Control Testing:

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis (FC): Staining of one million Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1064, Anti-Human CD3e (APA1/1) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^® Dye (Sold Separately)

標靶描述

T-cell surface glycoprotein CD3 epsilon chain (UniProt: P07766; also known as T-cell surface antigen T3/Leu-4 epsilon chain, CD3e) is encoded by the CD3E (also known as T3E) gene (Gene ID: 916) in human. CD3 is a single-pass type I membrane protein that is a part of the TCR-CD3 complex present on T-cells and plays an essential role in adaptive immune response. The TCR-CD3 complex is formed through noncovalent association of TCR with a CD3 signalling apparatus consisting of the  γ, δ, ε and ζ subunits. In addition of this role of signal transduction in T-cell activation, CD3ε plays an essential role in correct T-cell development. It participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3 cytosolic region. CD3ε is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-126), a transmembrane protein (aa 127-152), and a cytoplasmic domain (aa 153-207). The CD3 complex performs two major functions. Firstly, CD3 chains are required for intracellular signaling where tyrosines in its immunoreceptor tyrosine-based activation motif (ITAM) domain (aa 178-205) are phosphorylated by Lck. Secondly, the CD3 complex is involved in TCR surface expression. In the endoplasmic reticulum, the TCR heterodimer physically associate with the CD3 complex prior to moving to the Golgi for glycosylation and subsequently translocating to the T-cell surface. Mutations in CD3E gene are known to cause immunodeficiency 18 (IMD18) that is characterized by recurrent infections in infancy or early childhood with variable severity. (Ref.: Dong, D., et al. (2019). Nature. 573(7775); 546-552).

外觀

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重構

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律資訊

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable