應用
超氧阴离子检测试剂盒提供了一种对O2− 离子 细胞水平上的定量检测方法。该试剂盒可用于直接检测整个细胞在氧化胁迫下超氧阴离子的变化。此外，该试剂盒也可用于检测超氧化歧化酶（SOD）的活性。该试剂盒已被用于检测红细胞，白细胞和血浆中的SOD活性。
生化／生理作用
超氧阴离子 (O2-) 是一种短寿命自由基，通过氧分子上增加一个电子而产生。超氧阴离子的形成是对环境因素，如紫外光、香烟烟雾、环境污染物和γ射线等的响应，或由黄嘌呤氧化酶或NADPH氧化酶等氧化酶催化而产生。一旦形成， O2- 可攻击细胞租房引起脂质、蛋白质和DNA的损伤。它能引发多种疾病，包括癌症、动脉粥样硬化、类风湿关节炎、糖尿病、肝功能损伤，中枢神经系统紊乱。
分析報告
该试剂盒的方法是基于超氧阴离子氧化发光氨形成化学发光光线。该方法利用了一种特定的，非毒性物质能够放大化学发光信号。
仅试剂盒组分
产品编号
说明
- Assay Buffer 25 mL
- Assay Medium 20 mL
- Enhancer Solution, 40 mM 500 μL
- Luminol Solution 500 μL
- Xanthine Solution 500 μL
- Xanthine Oxidase .5 U
- Superoxide Dismutase from bovine liver, 4 units/μL 400 U
儲存類別代碼
10 - Combustible liquids
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