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CMC0006

Sigma-Aldrich

SIG10 ULTRA Electrocompetent Cells

for BAC & cosmid cloning

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.85

生物源

Escherichia coli

等級

for molecular biology

形狀

buffered aqueous solution

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: electrocompetent
transformation efficiency: ≥4 x 1010 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

The SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

特點和優勢

  • share the most useful genetic elements of standard cloning strains like DH5α DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

成分

  • SIG10 ULTRA electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

法律資訊

DH5α is a trademark of Invitrogen Corp.

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儲存類別代碼

10 - Combustible liquids


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