GM1899A

98120701, human unknown/unspecified, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: GM1899A, 98120701
• 生物源: human unknown/unspecified
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human lymphoblast, HPRT - deficient

細胞系描述

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

應用

Feeder for T-lymphocyte culture

培養基

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

其他說明

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