BHL-89

98070109, human lymph (axillary), Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: BHL-89, 98070109
• 生物源: human lymph (axillary)
• 增長模式: Suspension
• 染色體組型: Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: sIgM,CD22,BB1,HLA-ABC,GnRH
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human B-type EBNA negative lymphoma

細胞系描述

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

應用

B lymphoma studies, cancer treatments

培養基

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO₂; 37°C

其他說明

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