LB58

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: human blood
• 包裝: tube of 5 μg 98062325-DNA-5UG; pkg of vial of cells 98062325-1VL
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human lymphoblastoid, Ataxia telangiectasia, EBV-transformed

細胞系描述

Established by EBV transformation of mononuclear cells from the peripheral blood of a 13 year old female with Ataxia telangiectasia (AT) complementation group C. AT is a rare autosomal recessive disorder. Lymphocytes have been shown to be hypersensitive to cytotoxic and clastogenic effects of gamma and X rays. T cells from the same original sample have been used for studies of mutant frequency at the hprt locus, with cells being described as Donor 58 or Patient 2 (see Lancet 1985). Fibroblasts derived from the same individual were reported to have RNA and genomic DNA sequence changes (del, 9nt).

應用

hprt mutation studies, toxicity assays

培養基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

其他說明

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