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生物源
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髓瘤
Sp2/0
反應
Anti-Proteasomes, human
技術
cell culture | mammalian: suitable
同型
IgG1
運輸包裝
dry ice
儲存溫度
−196°C
相关类别
細胞系來源
Mouse Sp2/0 x Mouse BALB/c spleen
細胞系描述
Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.
免疫原
Human placenta proteasomes
應用
Detection and purification of proteasomes and analysis of subunits
培養基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO2; 37°C
其他說明
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