TGP 55

95062833, mouse pancreas, Polymorph

• 分類程式碼代碼: 41106514

属性

• product name: TGP 55, 95062833
• 生物源: mouse pancreas
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Polymorph
• 產品: Somatostatin and Insulin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse pancreatic small cell anaplastic carcinoma

細胞系描述

TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.

應用

Study of exocrine pancreas carcinomas, hormone secretion studies.

培養基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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