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生物源
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髓瘤
Sp2/5 (clone of SP2/0 Ag-14)
反應
Anti-Ricin β-chains
技術
cell culture | mammalian: suitable
同型
IgG1
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse Sp2/5 x Mouse BALB/c spleen immunised with ricin ß chain
細胞系描述
Produces a monoclonal antibody (IgG1) which reacts with the ß chain of the plant toxin ricin.
免疫原
Ricin ß chain
應用
Has been used in a protocol for purifying the α chains (A1 and A2) of ricin with minimal cross-contamination for the preparation of immunotoxins.
培養基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.
其他說明
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