C2-Rev 7

94101907, rat liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• 产品名称: C2-Rev 7, 94101907
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Hyperdiploid, 51 chromosomes
• 形態學: Epithelial
• 產品: Albumin and hepatocyte markers
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat hepatoma [HGPRT-]

細胞系描述

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

應用

Gene expression studies.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

其他說明

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