B16 melanoma 4A5 Cell Line from mouse

94042254, mouse skin, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• product name: B16 melanoma 4A5 Cell Line from mouse, Mouse melanoma, producing melanin, 94042254
• 生物源: mouse skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast-like
• 產品: Melanin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse melanoma, melanin producing

細胞系描述

Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.

應用

B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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