AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• 分類程式碼代碼: 41106514

属性

• 生物源: rat pancreas
• 包裝: tube of 5 μg 93100618-DNA-5UG; pkg of vial of cells 93100618-1VL
• 增長模式: Semi-adherent aggregates
• 染色體組型: Not specified
• 形態學: Pancreas cells
• 產品: Amylase and exocrine enzymes
• 受體: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat exocrine pancreatic tumour

細胞系描述

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

應用

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

其他說明

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