A15

93040110, rat brain, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: A15, 93040110
• 生物源: rat brain
• 增長模式: Adherent
• 染色體組型: Modal no. 42
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat, BDIX, glioma

細胞系描述

A renamed subculture of the rat gliomal line 1A2(R) - 93040129. A15 is tumourigenic in BDIX rats.

應用

Tumourigenicity studies

培養基

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm² flask using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C. Cells can reach up to 4x1,000,000 cells per 25cm² flask.

其他說明

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