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生物源
Pig x Pig Hybridoma
ImmunoDon
Boar spleen (d/d haplotype)
骨髓瘤
L231 (lymphoblastoid)
反應
Anti-Swine Testis (ST) cell antigen
技術
cell culture | mammalian: suitable
同型
IgA
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Pig L231 x boar spleen (d/d haplotype)
細胞系描述
The antibody is directed against the ST cell antigen as determined in virus enzyme linked cell immunoassays (VELCIA). The antibody does not cross-react with mouse 3T3 cells.
免疫原
Swine testis cell culture supernatant containing transmissible gastro-enteritis virus (strain Nouzilly 188.SG)
應用
VELICA, ELISA
培養基
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 2-10x100,000 cells/ml; 5% CO2; 37°C.
其他說明
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