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生物源
human blood
描述
gel volume in water M
HRC Panel 號碼
1
靜脈檢測年齡
61
種族
UK Caucasian
增長模式
Suspension
染色體組型
Not specified
形態學
Lymphoblastoid
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
相关类别
細胞系來源
UK Caucasian Male
細胞系描述
A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes. This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
應用
As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.
培養基
RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS). Once a growing culture is established the FBS concentration can be reduced to 10%.
例行更新培養
Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.
其他說明
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