HEPM

90120505, human palate epithelium. Established from palatal shelves.

• 分類程式碼代碼: 41106514

属性

• product name: HEPM, 90120505
• 生物源: human mouth (embryonic)
• 增長模式: Adherent
• 染色體組型: 2n = 46, diploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human embryonic palatal mesenchyme

細胞系描述

Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.

應用

Proliferation studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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