175BR

90011863, human skin, Fibroblast

• 分類程式碼代碼: 41106514

属性

• 产品名称: 175BR, 90011863
• 生物源: human skin
• 包裝: tube of 5 μg 90011863-DNA-5UG; pkg of vial of cells 90011863-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human skin fibroblast

細胞系描述

Established from an adult male with primary carcinoma. The donor was the brother of the donor of 174BR (Sigma Catalogue number. 90011895).

應用

DNA repair studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.