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生物源
Rat x Rat Hybridoma
ImmunoDon
Osborne-Mendel rat spleen
骨髓瘤
Y3Ag1.2.3
反應
Anti-p21 Protein, Harvey Murine Sarcoma Virus
技術
cell culture | mammalian: suitable
同型
IgG2a
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen
細胞系描述
The antibody is directed against the 21kDa transforming protein (p21) encoded by the v-ras gene of Harvey Murine Sarcoma Virus. It also reacts with the p21 encoded by BALB-MuSV and the 29kDa gag-ras fusion protein encoded by Ra-SV.
免疫原
Ha-MuSV transformed syngeneic NRK cells
培養基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask
其他說明
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