CCRF-SB

89090405, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: CCRF-SB, 89090405
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: 2n = 46
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian acute lymphoblastic leukaemia

細胞系描述

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

應用

HLA profile studies

培養基

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

其他說明

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