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product name
AK-D, 89071903
生物源
feline lung
增長模式
Adherent
染色體組型
Diploid
形態學
Epithelial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Foetal cat lung normal
細胞系描述
A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.
應用
Virus studies: panleucopenia, feline herpes and feline picornaviruses
培養基
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin and 0.03% EDTA; 5% CO2; 37°C.
其他說明
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