HL60 M2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: human blood
• 包裝: tube of 5 μg 88120803-DNA-5UG; pkg of vial of cells 88120803-1VL
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian promyelocytic leukaemia

細胞系描述

HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M2 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.

應用

Differentiation studies

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO₂; 37°C.

其他說明

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