ZR-75-30

88113004

• 分類程式碼代碼: 41106514

属性

• 生物源: human breast
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human breast carcinoma

細胞系描述

Derived from ascitic fluid of a 47 year old woman with infiltrating ductal carcinoma. Ethnicity: Black.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 10,11
D16S539: 9
D5S818: 12,13
D7S820: 11,12
THO1: 8,9
TPOX: 10,11
vWA: 18,19

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells produce a significant amount of cellular debris appearing as black particles. This cell line grows relatively slowly and requires passaging approximately every 5 days.

其他說明

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