P1.HTR.TK-

87121103, mouse breast, Mastocytoma

• 分類程式碼代碼: 41106514
• 生物源: mouse breast
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Mastocytoma
• 產品: Not specified
• 受體: Not specified
• 相關疾病: cancer

属性

• 产品名称: P1.HTR.TK-, 87121103
• 生物源: mouse breast
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Mastocytoma
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse DBA/2 mastocytoma

細胞系描述

Derived from the mouse mastocytoma P815 after serial cycles of transfection with a plasmid carrying a TK gene. BUdR is necessary in the growth media to avoid revertants.

應用

Genetic, transfection and tumourigenicity studies

培養基

DMEM + 2mM Glutamine + 100ug/ml Bromo-deoxy Uridine (BUdR) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 8% CO₂; 37°C. Media change twice a week.

其他說明

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