GH3

87012603, rat brain, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: GH3, 87012603
• 生物源: rat brain
• 增長模式: Adherent
• 染色體組型: Hyperdiploid, modal no. 60
• 形態學: Epithelial
• 產品: Growth hormone, prolactin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Wistar-Furth pituitary tumour

細胞系描述

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

應用

Hormone studies

培養基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

其他說明

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