CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: CRFK (BVD Ag negative), 86093002
• 生物源: feline kidney
• 增長模式: Adherent
• 染色體組型: Hypodiploid, modal no 36
• 形態學: Epithelial
• 產品: Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Cat kidney

細胞系描述

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

應用

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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