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生物源
mouse spleen
增長模式
Suspension
染色體組型
Not specified
形態學
Not specified
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse x Mouse myeloma, non-producing
細胞系描述
Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.
應用
Fusion partner
培養基
RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. HAT sensitive.
其他說明
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商品
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com
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