BHK 21 (Clone 13) from hamster

85011433, from hamster kidney

• 别名: BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: hamster kidney
• 增長模式: Adherent
• 染色體組型: 2n = 44, pseudodiploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Hamster Syrian kidney

細胞系描述

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

應用

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.

Virus replication studies

培養基

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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