VERO Cell Line

84113001, African green monkey kidney, Fibroblast-like

• 别名: Vero 81 Cells, VeroCCL81 Cells
• 分類程式碼代碼: 41106514

属性

• product name: VERO Cell Line, 84113001, from African green monkey kidney, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物源: African green monkey kidney
• 增長模式: Adherent
• 染色體組型: 2n = 60, modal no. 58
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

一般說明

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

細胞系來源

Monkey African Green kidney

細胞系描述

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

應用

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

培養基

(D6546) + 2 mM (G7513) + 10% (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他說明

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