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product name
MM466, 12070507
生物源
human skin (metastasis)
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
受體
Not specified
相關疾病
metastasis
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細胞系來源
Human melanoma metastatic site lymph node
細胞系描述
mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.
DNA分析
Not specified
培養基
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.
例行更新培養
Optimal split ratio 1:2-1:4 (seeding density 1.4 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.
其他說明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia
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