LIM2551

12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.

• 别名: LIM 2551, LIM-2551
• 分類程式碼代碼: 41106514

属性

• 产品名称: LIM2551, 12070505
• 生物源: human colon
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 形態學: Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
• 受體: Not specified
• 相關疾病: cancer

说明

細胞系來源

Human derived from an adenocarcinoma of the transverse colon

細胞系描述

LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.

DNA分析

Not specified

培養基

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.

例行更新培養

Optimal split ratio 1:8-1:16 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

其他說明

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia