108CC5-TG-4

8062546, Mouse x Rat Hybridoma nerve, Neuronal

• 分類程式碼代碼: 41106514

属性

• product name: 108CC5-TG-4, 08062546
• 生物源: Mouse x Rat Hybridoma nerve
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse neuroblastoma x Rat glioma hybrid

細胞系描述

A 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

應用

Used as a neuronal Model

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

其他說明

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