PNT1A (SERUM FREE)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 7052901, human prostate, Not specified

• 分類程式碼代碼: 41106514

属性

• 生物源: human prostate
• 包裝: tube of 5 μg 07052901-DNA-5UG; pkg of vial of cells 07052901-1VL
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human post pubertal normal, immortalised with SV40, serum-free

細胞系描述

This cell line is a derivative of the normal human prostate cell line PNT1A (Sigma cat no. 95012614) adapted to grow in serum-free medium. PNT1A was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem.

培養基

Serum-free Stemline Keratinocyte Medium II (Sigma cat no. S0196) + Keratinocyte Medium Supplement (Sigma cat no. K3136). To freeze cells use 50:50 fresh:conditioned medium + 10% DMSO

例行更新培養

Split sub-confluent cultures (70-80%) 1:2; 5% CO₂; 37 ºC. Mixture of attached and suspension cells. To harvest attached cells use gentle agitation. If cells do not detach, then try using 0.25% trypsin. A trypsin inhibitor must be used to inactivate trypsin. Seed contents of one ampoule into a 25 cm² flask.

其他說明

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