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产品名称
NS0-Turbodoma, 06020202
生物源
mouse blood
增長模式
Not specified
染色體組型
Not specified
形態學
lymphoblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse myeloma, serum-free, without cholesterol
細胞系描述
NS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.
培養基
Turbodoma medium (Cell Culture Technologies) + 2mM L-Glutamine (G7513). When freezing cells down use TurboDoma™ medium (50:50 conditioned medium:fresh medium) + 2mM L-Glutamine (G7513) + 10% DMSO.
例行更新培養
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
其他說明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法律資訊
TurboDoma is a trademark of Cell Culture Technologies
免責聲明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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