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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~100 kDa
物種活性
human
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~1.0 mg/mL
技術
western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B.
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CUL4B(8450)
mouse ... Cul4b(72584)
rat ... Cul4b(302502)
相关类别
一般說明
哺乳动物细胞表达两种不同但高度同源的cullin-4蛋白：cullin-4A和cullin-4B。Cul4A和Cul4B组成E3连接酶。与Cul4A相反，Cul4B位于细胞核。
應用
兔抗-Cullin-4B抗体适用于以表达人Cullin-4B的HEK-293T细胞在0.7-1.5 μg/mL的工作浓度下进行蛋白质印迹实验。在一项证明了CUL4B E3泛素连接酶在靶向Jab1（Jun激活结构域结合蛋白）进行降解并在调节骨形态发生蛋白信号转导中起关键作用的研究中，该抗体被用于免疫印迹。
生化／生理作用
Cullin-4 （Cul4）蛋白是调节细胞周期、基因组稳定性和DNA复制的泛素E3连接酶的核心成分。Cul4B可作为E3连接酶参与选择泛素化的特异性靶标。它与损伤特异性DNA结合蛋白 DDB1 和DDB2（紫外损伤DNA）有关，并可作为Cul4B E3 连接酶的底物募集因子（substrate-recruiting factor）。Cul4B 可使多种目标蛋白泛素化，包括β-连环蛋白、细胞周期蛋白E、组蛋白H2A。该基因的突变与X连锁智力低下有关。
Cullin-4B可使DNA损伤结合蛋白1 (DDB1) 无需依赖DDB2即可转移进入细胞核。与cullin-4A (Cul4A) 不同，cullin-4B (Cul4B) 不能泛素化DDB2。
標靶描述
Cullin-4B可形成一种能够充当E3泛素化连接酶的复合体，并催化细胞中特异性蛋白底物的多泛素化。该蛋白可与环指蛋白发生互作，并对多种调节因子的蛋白裂解是必需的
外觀
溶于含 15 mM 叠氮化钠的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 中。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Pengyu Li et al.
Oncology letters, 14(2), 1265-1274 (2017-08-16)
Cullin 4B (Cul4B), a scaffold protein that assembles the ubiquitin ligase complex, is involved in a wide variety of physiological and developmental processes, such as cell cycle progression, DNA damage response and gene expression regulation. Cul4B is overexpressed in various
The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells
Nag A, et al.
Molecular and Cellular Biology, 21(20), 6738-6747 (2001)
Rachana Tripathi et al.
Journal of biosciences, 32(6), 1133-1138 (2007-10-24)
Beta-catenin is the key transducer of Wingless-type MMTV integration site family member (Wnt) signalling, upregulation of which is the cause of cancer of the colon and other tissues. In the absence of Wnt signals, beta-catenin is targeted to ubiquitin-proteasome-mediated degradation.
Rachana Tripathi et al.
Journal of biosciences, 30(3), 329-337 (2005-07-30)
Heat induced differentiation of mouse embryonal carcinoma cells PCC4 has been reported earlier. We have further characterized the phenotype of the differentiated cells and by DD-RT-PCR identified several partial cDNAs that are differentially expressed during differentiation. Nucleotide homology search revealed
Yongxin Zou et al.
American journal of human genetics, 80(3), 561-566 (2007-02-03)
We reevaluated a previously reported family with an X-linked mental retardation syndrome and attempted to identify the underlying genetic defect. Screening of candidate genes in a 10-Mb region on Xq25 implicated CUL4B as the causative gene. CUL4B encodes a scaffold
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