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Merck
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Key Documents

C-41220

Sigma-Aldrich

DetachKit

3 x 250 ml

别名:

Cell Detachment Solution

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

包裝

pkg of 3 × 250 ml

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

DetachKit-250

應用

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

成分

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

品質

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

儲存和穩定性

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

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儲存類別代碼

10 - Combustible liquids


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