推荐产品
應用
牛红血球(BCA)来源碳酸酐酶已用于研究去除酶结合金属离子Zn2+对酶的聚集行为的影响。使用螯合剂（例如EDTA（乙二胺四乙酸） ）去除金属离子，增强了酶采用熔融球状态的倾向。人们发现，这种状态的酶可与分子伴侣样α-晶状体蛋白结合并防止聚集。来自Sigma的酶已固定在电化学传感器上，以便开发出彻底溶解CO2的分析装置。其还用于BCA构象变化的热力学分析。
生化／生理作用
碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶催化二氧化碳水合成碳酸。它参与重要的生理和病理过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致肿瘤性。因此，该酶是用于各种病理学（如青光眼、癫痫和帕金森病）的临床应用的抑制剂的重要靶标。
單位定義
在0°C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Heather N Hollowell et al.
Journal of biochemistry and molecular biology, 40(2), 205-211 (2007-03-31)
The stability curve - a plot of the Gibbs free energy of unfolding versus temperature - is calculated for bovine erythrocyte carbonic anhydrase in 150 mM sodium phosphate (pH = 7.0) from a combination of reversible differential scanning calorimetry measurements
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 165-171 (2019-11-23)
Testing of an expanded, 800-compound set of analogues of the earlier described Strecker-type α-aminonitriles (selected from publicly available Enamine Ltd. Screening Collection) in thermal shift assay against bovine carbonic anhydrase (bCA) led to further validation of this new class of
A J Boller et al.
Geobiology, 13(1), 33-43 (2014-10-11)
The cosmopolitan, bloom-forming diatom, Skeletonema costatum, is a prominent primary producer in coastal oceans, fixing CO2 with ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) that is phylogenetically distinct from terrestrial plant RubisCO. RubisCOs are subdivided into groups based on sequence similarity of their
Use of carbonic anhydrase in electrochemical biosensors for dissolved CO2
Cammaroto C, et al.
Sensors and Actuators B, Chemical, 48(1-3), 439-447 (1998)
K Rajaraman et al.
The Journal of biological chemistry, 271(44), 27595-27600 (1996-11-01)
alpha-Crystallin, a multimeric protein, exhibits chaperone-like activity in preventing aggregation of several proteins. We have studied the chaperone-like activity of alpha-crystallin toward heat-induced aggregation of bovine and human carbonic anhydrase. Human carbonic anhydrase aggregates at 60 degrees C, while bovine
实验方案
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门