C-39175

Small Airway Epithelial Cell Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• 别名: Recommended with C-21270
• 分類程式碼代碼: 12352207
• NACRES: NA.81

属性

• 形狀: aqueous solution
• 包裝: pkg of 1 vial
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Small Airway Epithelial Cell Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-21270

應用

Small Airway Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the distal respiratory system. The optimal adjustment of growth factors in this medium ensures excellent in vitro growth. The medium is optimized for primary human cells but can also be used for porcine small airway epithelial cells.Recommended for:Human Small Airway Epithelial Cells (HSAEpC)

成分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Small Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human small airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested small airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

準備報告

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin (recombinant human): 10 µg / mlRetinoic Acid: 0.1 ng / mlBovine Serum Albumin-Fatty Acid Free (BSA-FAF): 2.5 mg/ml

儲存和穩定性

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

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安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3