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Key Documents

C-39165

Sigma-Aldrich

Airway Epithelial Cell Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

别名:

Recommended with C-21260

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.81

形狀

aqueous solution

包裝

pkg of 1 vial

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Airway Epithelial Cell Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-21260

應用

Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages. The medium is optimized for primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells.Recommended for:Human Nasal Epithelial Cells (HNEpC)Human Bronchial Epithelial Cells (HBEpC)Human Tracheal Epithelial Cells (HTEpC)

成分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

準備報告

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin, holo (human): 10 µg / mlRetinoic Acid: 0.1 ng / ml

儲存和穩定性

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

Click here for more information.

推薦

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

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