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Key Documents

C3117

Sigma-Aldrich

Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

别名:

Monoclonal Anti-Cy3/Cy5

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

共軛

biotin conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

CY-96, monoclonal

形狀

buffered aqueous solution

濃度

~1 mg/mL

技術

direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable

同型

IgG2a

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

特異性

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

免疫原

mixture of proteins labeled with Cy3/Cy5.

應用

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • dot blot assay
  • protein microarrays
  • immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

生化/生理作用

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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