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共軛
(Glucosaminoglycan)
品質等級
形狀
lyophilized powder
比活性
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
成份
Protein, ~15% Lowry
溶解度
0.02 M phosphate buffer: soluble (pH 7.0)
異物活動
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
儲存溫度
−20°C
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應用
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
生化／生理作用
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
其他說明
Contains potassium phosphate buffer salts and BSA as stabilizer.
查看有关 用于复合碳水化合物分析的酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
適合性
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
單位定義
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
準備報告
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Florian D Naal et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 87(1), 19-25 (2008-03-25)
Meniscal allograft processing procedures, in particular gamma irradiation, deteriorate the biomechanical and biological properties of the transplanted tissue. High hydrostatic pressure (HHP) treatment, widely used in food technology to inactivate microorganisms while preserving natural compounds, might serve as a gentle
Jessica S Kim et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(8), 780-790 (2011-08-02)
Cutaneous lupus erythematosus and dermatomyositis (DM) are chronic inflammatory diseases of the skin with accumulated dermal mucin. Earlier work has shown chondroitin sulfate (CS) accumulation within the dermis of discoid lupus erythematosus (DLE), subacute cutaneous lupus erythematosus (SCLE), and DM
E B Hunziker
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We have previously shown (Hunziker and Rosenberg, J Bone Joint Surg 1996;78A:721-33) that synovial cells can be induced to migrate into partial-thickness articular cartilage defects, therein to proliferate and subsequently to deposit a scar-like tissue. We now wished to ascertain
C B Knudson et al.
The Journal of cell biology, 100(5), 1753-1758 (1985-05-01)
Hyaluronate levels change dramatically during morphogenesis of various tissues and organs. Morphological detection of the exact temporal and spatial distribution patterns of hyaluronate may help to elucidate its role in morphogenesis. Since no specific direct method for visualizing hyaluronate with
S P Levine et al.
Blood, 75(4), 902-910 (1990-02-15)
Platelet factor 4 (PF4) is a hydrophobic, alpha-granule protein with potent antiheparin activity. It also binds to a chondroitin sulfate-containing proteoglycan (PG) isolated from platelets. In order to evaluate further the relationship between PF4 and the chondroitin sulfate-containing proteoglycan in
商品
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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