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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
hCP, monoclonal
分子量
antigen 34 kDa
包含
15 mM sodium azide
物種活性
rat, rabbit, pig, human, mouse
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000 using human uterus extract
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CNN1(1264) , CNN2(1265)
mouse ... Cnn1(12797) , Cnn2(12798)
rat ... Cnn1(65204) , Kcnip4(259243)
特異性
hCP抗体（又称CALP）可定位哺乳动物平滑肌中的钙调蛋白。在人子宫中，一种约 27 kDa 的附加条带(1-钙调蛋白)也可能随之染色。该抗体不会与骨骼肌、心脏或非肌肉组织的钙调蛋白发生交叉反应。然而，该抗体与人或小鼠骨骼肌中的表位(150 kDa 范围)表现出交叉反应。在免疫组化染色中，该产品表现出平滑肌特异性。它可以染色组织切片和原代培养细胞(或早期传代细胞)中的血管和内脏平滑肌细胞，但不能染色大多数最初源自平滑肌的细胞系。该抗体不能染色上皮、内皮或结缔组织成纤维细胞。 该产品不与鸡肉的平滑肌组织发生交叉反应。
免疫原
人子宫平滑肌提取物。
應用
小鼠产生的抗-钙调蛋白单克隆抗体适用于以下应用：
- 使用福尔马林固定、石蜡包埋切片的免疫组化
- 免疫沉淀
- 微阵列
- 蛋白质印迹（使用人子宫提取物，稀释度为 1:10,000）
- 流式细胞仪分析
生化／生理作用
钙调蛋白是钙结合蛋白，属于肌动蛋白相关蛋白家族。钙调蛋白是血管平滑肌(VSM)中蛋白激酶 C(PKC)自磷酸化所必需的。钙调蛋白-1 抑制可防止子宫平滑肌细胞迁移、引起形态变化和F-肌动蛋白重排，而不会影响其增殖和凋亡。钙调蛋白 H1(CN)是平滑肌细胞的分化标志物，在几种人类肿瘤的血管中下调。它可以抑制肌动球蛋白 ATP 酶活性。h1 和 h2 钙调蛋白与 F-肌动蛋白结合，并在调节平滑肌和非肌肉细胞的肌动蛋白丝中发挥关键作用。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Yong-hong Gu et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 30(6), 1369-1372 (2010-06-30)
To study the effects of calponin-1 expression inhibition on the proliferation , invasiveness, apoptosis and cytoskeleton of uterine smooth muscle cells, and explore the molecular mechanism of calponin-1 in the uterine smooth muscle cells for labor onset. siRNA-calponin-1 adenovirus plasmid
Guoqing Hu et al.
The Journal of biological chemistry, 285(16), 11800-11809 (2010-02-20)
Fully differentiated mature smooth muscle cells (SMCs) are characterized by the presence of a unique repertoire of smooth muscle-specific proteins. Although previous studies have shown myocardin to be a critical transcription factor for stimulating expression of smooth muscle-specific genes, the
Jian-Ping Jin et al.
Protein expression and purification, 31(2), 231-239 (2003-10-11)
Three homologous calponin isoforms, named h1, h2, and acidic calponins, have been found in birds and mammals. Based primarily on studies of chicken gizzard smooth muscle (h1) calponin, calponin has been identified as a family of actin-associated proteins that inhibit
Xiaoke Yin et al.
Proteomics, 6(24), 6437-6446 (2006-12-14)
Embryonic stem (ES) cells can differentiate into vascular smooth muscle cells (SMCs), but differences in protein composition, function and behaviour between stem cell-derived and mature SMCs remain to be characterized. Using differential in gel electrophoresis (DIGE) and MS, we identified
Y Yanagisawa et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 34(5), 531-537 (2007-08-21)
Calponin h1 (CN) is a differentiation marker of smooth muscle cells that has been reported to be down-regulated in the blood vessels of several human tumors. In this study, we examined CN expression in blood vessels in relation to the
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