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生物源
human bone (knee and hip joints)
包裝
pkg of 500,000 cells
形態學
(osteoblasts)
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
一般說明
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
細胞系來源
Bone Tissue
應用
Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request) and are provided in a cryopreserved format. They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid. During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.
品質
Rigid quality control tests are performed for each lot of Human Osteoblasts. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, histochemical tests for alkaline phosphatase and bone mineralization are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions lacking antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
警告
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
例行更新培養
Click here for more information.
其他說明
Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positive, Mineralization testedGuaranteed population doublings: >10
推薦產品
Recommended Primary Cell Culture Media:Link
免責聲明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Ilaria Lorenzon et al.
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商品
Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.
Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.
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